Kastamonu Medical Journal regularly publishes internationally qualified issues in the field of Medicine in the light of up-to-date information.

Original Article
Effects of exercise at the anaerobic threshold on respiratory quotient in young male subjects
Aims: Exercise has great influence on increasing metabolic system functions. The work load corresponded to anaerobic threshold provide optimal aerobic strain for metabolic activity in exercising muscle. In the present study we intended to evaluate body substrate oxidation ratio during constant load exercise test at the intensity of anaerobic threshold in healthy young male subjects.
Methods: Total of 15 male performed an incremental ramp exercise test to estimate anaerobic threshold. Standard V-slope method used to estimate anaerobic threshold. Then each subjects performed a constant load exercise test for a 30 min period with a work load corresponded to their anaerobic threshold. Respiratory quotient (RQ) used to evaluate substrate oxidations during exercise. Anova test used to evaluate significance of data obtained every 5 minutes of constant load exercise.
Results: The subjects’ anaerobic threshold occurred at approximately 63% of their maximal exercise capacity. RQ varied markedly among the subjects but as a mean values, but it systematically decreased with increasing exercise time. Body mass index and exercise time has great importance on fat and carbohydrate oxidation ratio.
Conclusion: Exercise intensity at the anaerobic threshold provides meaningfully fat oxidation and could be acceptable in subjects with high body fat mass.

1. Palange P, Ward SA, Carlsen KH, et al. Recommendations on the use of exercise testing in clinical practice. EurRespir J. 2007;29:185-209.
2. Wasserman K, Hansen JE, Sue DY, et al. Principles of exercise testing and interpretation: including pathophysiology and clinical applications. 5. Edition, Philadelphia: Lippincott Williams & Wilkins, 2012.
3. Kelly RS, Kelly MP, Kelly P. Metabolomics, physical activity, exercise and health: A review of the current evidence. Biochim Biophys Acta Mol Basis Dis 2020;1866:165936.
4. Wang X, Lyles MF, You T, Berry MJ, Rejeski WJ, Nicklas BJ. Weight regain is related to decreases in physical activity during weight loss. Med Sci Sports Exerc 2008;40:1781-8.
5. Ozcelik O, Ozkan Y, Algul S, Colak R. <a href="javascript:void(0)">Beneficial effects of training at the anaerobic threshold in addition to pharmacotherapy on weight loss, body composition, and exercise performance in women with obesity</a>. Patient Preference and Adherence 2015; 9: 999-1004-.
6. Friedenreich CM, Ruan Y, Duha A, Courneya KS. Exercise dose effects on body fat 12 months after an exercise intervention: follow-up from a randomized controlled trial. J Obes 2019;2019:3916416.
7. Hunter GR, Fisher G, Neumeier WH, Carter SJ, Plaisance EP. Exercise training and energy expenditure following weight loss. Med Sci Sports Exerc 2015;47:1950-7.
8. Rynders CA, Blanc S, DeJong N, Bessesen DH, Bergouignan A. Sedentary behaviour is a key determinant of metabolic inflexibility. J Physiol. 2018;596:1319-30.
9. J&uacute;dice PB, Sardinha LB, Silva AM. Variance in respiratory quotient among daily activities and its association with obesity status. Int J Obes (Lond) 2021;45:217-24.
10. Spurway NC. Aerobic exercise, anaerobic exercise and the lactate threshold. Br Med Bull 1992;48:569-91.
11. Ugras S, Algul S, Ozcelik O. <a href="javascript:void(0)">Assessing the effectiveness of anaerobic threshold and respiratory compensation point on fat and carbohydrate oxidations during exercise in sedentary males</a>. Progress in Nutrition 2020; 22: 4.e2020078.
12. Emerenziani GP, Ferrari D, Marocco C, et al. Relationship between individual ventilatory threshold and maximal fat oxidation (MFO) over different obesity classes in women. PLoS One 2019;14:e0215307.
13. Ugras S. Evaluating of altered hydration status on effectiveness of body composition analysis using bioelectric impedance analysis. Libyan J Med 2020;15:1741904.
14. Whipp BJ, Davis JA, Torres F, Wasserman K. A test to determine parameters of aerobic function during exercise. J ApplPhysiolRespir Environ ExercPhysiol 1981;50:217-21.
15. Ozcelik O, Ward SA, Whipp BJ. Effect of altered body CO2 stores on pulmonary gas exchange dynamics during incremental exercise in humans. ExpPhysiol 1999;84:999-1011.
16. Beaver WL, Wasserman K, Whipp BJ. A new method for detecting anaerobic threshold by gas exchange J ApplPhysiol 1986; 60: 2020-7.
17. Whipp BJ, Ward SA, Wasserman K. Respiratory markers of the anaerobic threshold.AdvCardiol 1986;35:47-64.
18. Algul S, Ugur FA, Ayar A, Ozcelik O. Comparative determination of ventilatory efficiency from constant load and incremental exercise testing. Cell MolBiol (Noisy-le-grand) 2017;63:26-30.
19. Broskey NT, Pories WJ, Jones TE, et al. The association between lactate and muscle aerobic substrate oxidation: Is lactate an early marker for metabolic disease in healthy subjects? Physiol Rep 2021;9:e14729.
20. Uğraş S, &Ouml;z&ccedil;elik O. <a href="javascript:void(0)">Eşik altı yoğunluğundaki egzersizin gen&ccedil; erkeklerde v&uuml;cut yağ ve karbonhidrat yakım miktari ve oranı &uuml;zerine olan etkileri.</a>Genel Tıp Dergisi2019; 29: 48-54.
21. Ozcelik O, Aslan M, Ayar A, Kelestimur H. Effects of body mass index on maximal work production capacity and aerobic fitness during incremental exercise. Physiol Res 2004;53:165-70.
22. Shook RP, Hand GA, Paluch AE, et al. High respiratory quotient is associated with increases in body weight and fat mass in young adults. Eur J ClinNutr 2016;70:1197-202.
23. &Ccedil;olak R, &Ouml;z&ccedil;elik O. Effects of progressively increasing work rate exercise on body substrate utilisation. Turk J EndocrinMetab 2002; 2: 81-4.
24. Ozcelik O, Dogan H, Kelestimur H. Effects of acute hypoxia on body substrate utilisation during progressively increasing work rate exercise tests.Turk J Med Sci2003;33: 223-8.
25. J&uacute;dice PB, Sardinha LB, Silva AM. Variance in respiratory quotient among daily activities and its association with obesity status. Int J Obes (Lond) 2021;45:217-24.
26. deBeaudrap P, Witten G, Biltz G, Perrier E. Mechanistic model of fuel selection in the muscle. J Theor Biol 2006;242:151-63.
27. Goodpaster BH, Sparks LM. Metabolic flexibility in health and disease. Cell Metab2017; 25: 1027-36.
Volume 1, Issue 1, 2021
Page : 9-12