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Kastamonu Medical Journal

Kastamonu Medical Journal regularly publishes internationally qualified issues in the field of Medicine in the light of up-to-date information.

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Original Article
Effect of lipoic acid on cisplatin-induced cardiotoxicity and inflammation in cardiomyocytes
Abstract
Aims: Cisplatin (CPL) is a potent chemotherapeutic agent widely used to treat various cancers. However, CPL causes toxicity in various tissues, including the heart. Lipoic acid (LPA) is a thiol compound with antioxidant, anti-inflammatory, and antiapoptotic properties. Although LPA has been reported to have protective effects in various cardiac diseases, the mechanisms underlying its cardio-protective effects have not been elucidated. This study aimed to establish a CPL-induced cardiotoxicity model in the H9c2 cardiomyocyte cell line, to understand the mechanisms underlying this cardiotoxicity, and to investigate the effect of LPA on cardiotoxicity.
Methods: H9c2 cardiomyocyte cells as control (CNT), CPL (40 µm), and LPA-1 (300 µm LPA, and 40 µm CPL), LPA-2 (500 µm LPA, and 40 µm CPL) in combination along with CIS were used. In the analyses made, glutathione (GSH) and glutathione peroxidase (GSHPx) enzyme activity, lipid peroxidation [malondialdehyde, (MDA)] levels, inflammation markers interleukin (IL) -1ß, IL-6, and tumor necrosis factor (TNF) -? levels, total oxidant/antioxidant (TOS and TAS) status levels, reactive oxygen species (ROS) and caspase activity (Casp 3 and 9) in the cells were determined.
Results: CIS treatment caused cardiomyocyte cell toxicity and increased Casp 3, Casp 9, ROS, IL-1ß, IL-6, TNF-?, TOS, and MDA levels while decreasing GSHPx, GSH, and TAS levels. Increased inflammation and impaired oxidant/antioxidant balance in cardiomyocyte cells after CPL treatment were regulated by LPA treatment.
Conclusion: LPA treatment was found to have a protective effect against CPL-induced cardiotoxicity in cardiomyocyte cells.

Keywords : Apoptosis, lipoic acid, cardiotoxicity, cisplatin, oxidative stress

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Article available in :
Volume 4, Issue 4, 2024
Page : 175-181
https://doi.org/10.51271/KMJ-0167
Article Information
Received : 12 Ağu 2024
Accepted : 03 Eyl 2024
Published : 01 Ara 2024
Corresponding Author :

Yener Yazğan: Department of Biophysics, Faculty of Medicine, Kastamonu University, Kastamonu, Türkiye

[email protected]
Citation : Yazğan Y, Çınar R, Yazğan B. Effect of lipoic acid on cisplatin-induced cardiotoxicity and inflammation in cardiomyocytes. Kastamonu Med J. 2024;4(4):175-181.

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