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Kastamonu Medical Journal

Kastamonu Medical Journal regularly publishes internationally qualified issues in the field of Medicine in the light of up-to-date information.

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Original Article
The protective effect of chrysin against glutamate-induced oxidative stress and inflammation in SH-SY5Y cells
Abstract
Aims: Chrysin is a flavonoid with economic value and medicinal effects commonly found in propolis, honey, and blue passion flowers. It has many pharmacological properties such as anticancer, antitumor, antioxidant, neurotrophic, and antidepressant. Glutamate plays an important role in brain functions; however, its high concentration in the central nervous system causes neurotoxic effects. This study aimed to investigate the effect of Chrysin on glutamate-induced cytotoxicity in SH-SY5Y neuroblastoma cells.
Methods: This study was conducted in 4 groups; control, Chrysin (50 µM), glutamate (10 mM), and glutamate+Chrysin. cell viability, total oxidant status (TOS), total antioxidant status (TAS), tumor necrosis factor alpha (TNF-a), and interleukin 1 ß (IL-1ß) levels in the cells were determined by ELISA kit.
Results: It is shown that glutamate application caused cytotoxicity in SH-SY5Y neuroblastoma cells and increased TOS, TNF-a, and IL-1ß levels. However, in SH-SY5Y cells treated with Chrysin before glutamate incubation, TOS, TNF-a, and IL-1ß levels decreased compared to the glutamate group, while TAS and cell viability levels increased.
Conclusion: Chrysin’s antioxidant properties played a protective role in SH-SY5Y cells against glutamate-induced increased oxidative stress and inflammation.

Keywords : Anti-inflammatory, chrysin, glutamate, oxidative stress, SH-SY5Y cells

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Article available in :
Volume 5, Issue 3, 2025
Page : 166-171
https://doi.org/10.51271/KMJ-0204
Article Information
Received : 29 Kas 2024
Accepted : 05 Mar 2025
Published : 03 Eyl 2025
Corresponding Author :

Yener Yazğan: Department of Biophysics, Faculty of Medicine, Kastamonu University, Kastamonu, Turkiye

[email protected]
Citation : Ahlatcı A, Yazğan Y, Yıldızhan K. The protective effect of Chrysin against glutamate-induced oxidative stress and inflammation in SH-SY5Y cells. Kastamonu Med J. 2025;5(3):166-171.

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