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Kastamonu Medical Journal

Kastamonu Medical Journal regularly publishes internationally qualified issues in the field of Medicine in the light of up-to-date information.

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Original Article
The effect of minocycline in suppressing TGF-B1 expression to reduce the formation of glial scar in traumatic brain injury model rats
Abstract
Aims: Traumatic brain injury (TBI) is a long-term disorder that results in lifelong disability, often in the form of cognitive impairment due to glial scar formation. Treatment with minocycline may reduce the Transforming Growth Factor-Beta-1 (TGF-ß1) pathway. Our assumption was that minocycline also contributed to the inhibition of glial scar via the TGF-ß1 pathway in TBI. The study aimed to assess how minocycline affects the TGF-ß1 signaling pathway in TBI model rats to inhibit the formation of glial scars.
Methods: Twenty male Sprague Dawley rats were divided in five groups, with four rats per group. Day 0 of TBI using the weight drop model was followed by 14 days of minocycline treatment at doses of 25 mg/kg (MNO1 group), 50 mg/kg (MNO2 group), and 100 mg/kg (MNO3 group). On day 15, the Novel Object Recognition (NOR) test was performed. On day 16, immunofluorescence double staining was carried out.
Results: In rats with TBI models, minocycline inhibited the formation of glial scars. It has been demonstrated that minocycline inhibits the formation of TGF-ß1, which in effect inhibits the formation of glial scar in the perilesional area of TBI model rats. In TBI model rats, inhibition of glial scar may ameliorate cognitive function deficit. In TBI model rats, minocycline administration can improve cognitive function.
Conclusion: Through the reduction of TGF-ß1 expression, minocycline inhibited glial scar formation in a rat model of TBI.

Keywords : Glial scar, minocycline, rats, TGF-ß1, traumatic brain injury

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Article available in :
Volume 5, Issue 4, 2025
Page : 253-258
https://doi.org/10.51271/KMJ-0221
Article Information
Received : 08 Nis 2025
Accepted : 13 Tem 2025
Published : 02 Ara 2025
Corresponding Author :

Donny Wisnu Wardhana: Division of Neurosurgery, Departement of Surgery, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia

[email protected]
Citation : Wardhana DW, Khotimah H, Nazwar TA, Nurdiana N. The effect of minocycline in suppressing TGF-?1 expression to reduce the formation of glial scar in traumatic brain injury model rats. Kastamonu Med J. 2025;5(4):253-258.

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